Pluto Bioinformatics

GSE128466: Eomes and Brachyury control pluripotency exit and germ-layer segregation by changing the chromatin state

Bulk RNA sequencing

We aimed to elucidate molecular mechanisms of first lineage decision in the mouse pluripotent epiblast that segregates mesoderm and endoderm (ME) from neuroectoderm (NE). By analyzing mouse embryonic stem cells (ESCs) and embryos deficient for two T-box (Tbx) transcription factors Eomes and Brachyury we demonstrate that this process is controlled by the changes in the chromatin accessibility of ME gene enhancers and activation of the target genes, but also direct repression the opposing pluripotency and NE gene programs. SOURCE: Sebastian,J.,Arnold (sebastian.arnold@pharmakol.uni-freiburg.de) - Regenerative Pharmacology University of Freiburg