Pluto Bioinformatics

GSE102132: KSHV vIRF3 promotes angiogenesis of lymphatic endothelial cells (LECs)

Bulk RNA sequencing

Herein, we report viral interferon regulatory factor 3 (vIRF3) as a pro-angiogenic factor together with HDAC5 that coordinates with LEC transcriptome, thus resulting in hyper-sprouting formation and ultimately leading to abnormal lymphangiogenesis. Overexpression of vIRF3 in LECs but not blood endothelial cells (BECs) exhibited a unique KSHV-associated angiogenic effect, evidenced by elongated spindle shape, sprouting, and tube formation. Furthermore, using vIRF3 knockout recombinant KSHV, we confirmed that vIRF3 is a critical factor of KSHV-mediated lymphangiogenesis in primary LECs. SOURCE: Fan Li (fli@chla.usc.edu) - SC2 Core Children's Hospital Los Angeles