Pluto Bioinformatics

GSE132433: Estrogen response in breast cancer cell line MCF-7 is dependent on NR2F2 [RNA-seq]

Bulk RNA sequencing

We show that most binding events of NR2F2 occur together with the ER binding sites.To address the functional relationship between NR2F2 and ER, we assessed the role of NR2F2 in oestrogen-induced growth in ER positive cell line MCF-7. The MTT experiment showed that inhibition of NR2F2 prevented the oestrogen-induced proliferation of MCF-7 cells.To further explore the effect of NR2F2 on estrogen response, We expanded our knockdown studies by performing RNA-seq analysis for MCF-7 cells transfected with control or NR2F2 shRNAs with or without E2. SOURCE: Guojuan Jiang (jgj5292@126.com) - State Key Laboratory of Medical Genomics Shanghai Jiaotong University