Metabolic engagement is intrinsic to immune cell function. Prostaglandin E2 (PGE2) has been shown to modulate macrophage activation, yet how PGE2 might affect metabolism is unclear. Here we show that PGE2 causes mitochondrial membrane potential (m) to dissipate in interleukin-4 activated macrophages (M(IL-4)). Effects on m are a consequence of PGE2-initiated transcriptional regulation of genes in the malate-aspartate shuttle (MAS), particularly GOT1. Reduced m causes alterations in the expression of 126 voltage regulated genes (VRGs) including Resistin like molecule- (RELM), a key marker of M(IL-4), and genes that regulate cell cycle. The transcription factor ETS variant 1 (ETV1) plays a role in the regulation of 38% of the VRGs. These results reveal ETV1 as a m-sensitive transcription factor, and m as a mediator of mitochondrial-directed nuclear gene expression. SOURCE: David,E,Sanin (sanin@ie-freiburg.mpg.de) - Pearce Max-Planck-Institute für Immunbiologie und Epigenetik

Pluto Bioinformatics

GSE119509: Mitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to PGE2 (RNA-seq)