Pluto Bioinformatics

GSE150924: YBX1 indirectly targets heterochromatin-repressed inflammatory response genes through binding to CBX5 [RNA-Seq]

Bulk RNA sequencing

The RNA- and DNA-binding protein YBX1 is involved in various cellular processes. We here focused on investigating its role in posttranscriptional gene regulation in medulloblastoma, in which CRISPR screens have shown its essentiality for cellular survival. We found that YBX1 knockdown leads to broad upregulation of genes related to neuroinflammation, and directly regulates mRNA stability of various mRNA targets. More specifically, target CBX5, also called heterochromatin-protein 1 alpha (HP1-alpha), is downregulated upon YBX1 KD. It associates strongly with heterochromatin, and upon downregulation allows for upregulation of heterochromatin repressed genes related to inflammation. SOURCE: Andreas Kloetgen NYU School of Medicine