Pluto Bioinformatics

GSE108388: Systematic functional characterization of BAF mutations yields novel intra-complex synthetic lethalities [RNA-Seq]

Bulk RNA sequencing

Here we develop and characterize an isogenic cell line model consisting of knock-out clones for 22 targetable BAF subunits. In these cell lines, we characterize the effects of loss of individual subunits on the complex composition on the proteomic level, on gene expression, and on chromatin accessibility. Finally, we systematically analyze all intra-complex synthetic lethalities and integrate all data to provide a molecular rationale for targeting BAF mutant cancers. SOURCE: Christoph Bock (cbock@cemm.oeaw.ac.at) - CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences