Pluto Bioinformatics

GSE79169: Determine the function of lncBATE10 in brown and white adipocyte culture

Bulk RNA sequencing

Purpose: Determine the function of lncBATE10 in brown and white adipocyte differentiation; Methods: primary brown and white apreadipocytes were isolated by cell culture. We infected brown preadipocytes with retroviral shRNA targeting lncBATE10 or with retrovirus overexpressing lncBATE10. Cells were induced to differetinate for 5 days. Total RNA were harvested for RNA-seq; Conclusions: Our study shows that lncBATE10 is required for BAT-selective program expression. Overexpression of lncBATE10 is not sufficient to promote BAT marker expression. SOURCE: Xiaoran Chai (chaix@gis.a-star.edu.sg) - Genome Institute of Singapore