Purpose: The goal of present study is to compare transcript level changes between normal and tumor of same individual;	Methods: Total RNA was isolated, strand specific RNA seq was performed after Ribosomal RNA removal, global transcript profiles were generated by deep sequencing, in duplicate, using Illumina Hiseq. Initial quality check was performed using FASTQC, Alignment was performed using Hisat2 against human hg19 genome. Raw read count was performed using SummarizedExperiment (R package). Differential analysis was done using DEseq2;	Results: This study provides insights in terms of global trancriptome changes between normal and tumor of same patient.;	Conclusions: This study provides in depth analysis of transcriptomoc data in patient specific manner. SOURCE: Hinrich Gronemeyer (hg@igbmc.fr) -  IGBMC

Pluto Bioinformatics

GSE133626: Genome wide transcriptome analysis of patient matched prostate cancer using NGS technology