Pluto Bioinformatics

GSE92434: RNA- and ATAC-seq data of Wt, Ebf1-KO and Pax5-KO FL-ProB-cells.

Bulk RNA sequencing

EBF1 and PAX5 are crucial factors for B-cell development. Knock down of either factor leads to an arrest in differentiation. RNA-seq data of WT, Ebf1-/- and Pax5-/- FL-ProB cells allowed for identification of activated and repressed target genes. ATAC-seq data of corresponding cell populations could connect gene activity and chromatin accessibility. This finding provided an explanation to the lineage instability observed in early B-cell progenitors. Taken together, the reported data provide an increased insight into mechanisms governing regulation of stage- and lineage specific transcription in early B-lymphocyte development. SOURCE: Mikael Sigvardsson (mikael.sigvardsson@med.lu.se) - Sigvardsson Lund University