Pluto Bioinformatics

GSE99399: A transcriptomic study of myogenic differentiation under the overexpression of PPAR by RNA-Seq

Bulk RNA sequencing

To study the cellular and molecular function of PPAR in skeletal muscle differentiation, we have generated inducible gain-of-function to overexpress PPAR in C2C12 skeletal muscle cells. In order to identify PPAR targets, RNA sequencing (RNA-seq) was used to evaluate and quantify of transcriptomes and expression patterns during myogenic differentiation under the overexpression of PPAR. The formation of myotubes and the expression of muscle-specific myogenic genes such as MyoD and MyoG may be inhibited by any altered expression of PPAR. Multiple genes and pathways were significantly involved in this process, including 11 genes such as Fndc9 and Slc14a1 with fundamental change of regulation modes, 9 genes of which were validated by the data of qRT-PCR. Our studies demonstrate that PPAR would play critical roles on the skeletal muscle cells differentiation, mediating crosstalk among several pathways and transcription factors. SOURCE: Kan He (hekan803@hkbu.edu.hk) - Anhui University