Pluto Bioinformatics

GSE108921: Gene Expression Profiling of WT and KDM3A Knocked out Cell

Bulk RNA sequencing

We identified KDM3A, a demethylase of histone H3K9me1/2, as a positive regulator for hippo target genes. We found that H3K27ac upregulation is highly correlated with gene activation, but not H3K4me3; and transcription repression of certain TEAD1 target genes, such as BBC3, is important for the pathway function. KDM3A knockout caused upregulation of H3K9me2 mainly on TEAD1-binding enhancers rather than gene bodies, leading to decrease of H3K27ac and TEAD1 binding on enhancers and impaired transcription. SOURCE: Pinji Lei (leipinji@gmail.com) - Epigenomics Wuhan University