Pluto Bioinformatics

GSE76857: Direct Induction of Neural Progenitor Cells Transiently Passes Through a Partially-reprogrammed State

Bulk RNA sequencing

During giNPC induction process, cell samples were harvested at various induction days including named Day1, Day4, Day7, Day10, Day14, Day17 and Day21. Meanwhile, samples from primary neurosphere-like cluster (named pre-NPCs), established giNPC cell line at passage 6 (named giNPCs P6), Brain-NPCs as well as Teratoma were also collected. Total RNA was isolated from cell pellets by Trizol reagent and RNA-Seq library were generated using KAPA Strandard mRNA-Seq Kits according to the manufacturer's recommendations. SOURCE: Shaorong Gao (gaoshaorong@tongji.edu.cn) - Tongji University