Pluto Bioinformatics

GSE57514: Transcriptome profile of oocytes expressing a catalytically inactive knock-in allele of Ago2

Bulk RNA sequencing

The RNase III enzyme DICER generates both microRNAs (miRNAs) and endogenous short interfering RNAs (endo-siRNAs). Dicer deletion in mouse oocytes leads to female infertility due to defects during meiosis I. Because miRNA function is suppressed in mouse oocytes, it has been proposed that endo-siRNAs may have a role during female meiosis. By utilizing a catalytically inactive knock-in allele of AGO2 specifically in oocytes, we disrupt the function of siRNAs and analyze the transcriptome of these oocytes in comparison with Ago2 null, and Dicer null oocytes. SOURCE: Paula Stein (steinpau@sas.upenn.edu) - Richard Schultz University of Pennsylvania