Pluto Bioinformatics

GSE81576: Gene expression profiling of Eed knockout and Ezh2 knockout small intestinal crypts [RNA-seq]

Bulk RNA sequencing

Polycomb-mediated gene repression plays an important role in adult stem cell maintenance. We knocked out (using the inducible AhCre-LoxP system) Polycomb genes Eed and Ezh2 in the intestine for 6 weeks, after which crypts - the small intestinal stem cell zone - were harvested and RNA sequenced. We found Wnt, Notch and cell cycle pathways to be affected in Eed knockout (KO) but not Ezh2 KO crypts. Direct targets of Eed were determined by comparing this data with ChIP-sequencing. SOURCE: Martijn Koppens (majkoppens@gmail.com) - NKI-AVL