Pluto Bioinformatics

GSE111118: mRNA profiles of active hematopoieitc stem cells

Bulk RNA sequencing

Purpose: The goals of this study are to elucidate the underlying mechanism for the activation of HSCs; Methods: After wild type (WT) HSCs (CD150+ CD48- EPCR+ Lineage-) were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after the culture with or without Nifedipien, cultured HSC fraction (CD150+ CD48- EPCR+ c-kit+ Sca-1+ Lineage-) were sorted. These cells were subjected to mRNA sequence using Next-seq (n>3). The sequence reads that passed quality filters were analyzed by CLC genomic workbench. SOURCE: Terumasa Umemoto Kumamoto University

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