Bidirectional transcription is a prevalent feature of eukaryotic promoters. The goal of this study is to identify novel divergent long-coding RNAs during differentiation of human ES to cardiomyocytes.;	Methods: Paired end RNA-Seq was performed on key time-points of human ES cell differentiation to cardiomyocytes. The time-points include  day2, day4, day6 and day8.;	Results: Using an optimized data analysis workflow, we mapped about ~70 million sequence reads per sample to the human genome (build hg38) and identified 781 novel yylncRNAs during ES cell differentiation to cardiomyocytes. Expression kinetics of a subset of  yylncRNAs identified from RNA-Seq  had a linear relationship with qRTPCR .;	Conclusions: Our study represents the detailed analysis of the genome wide identification of divergent long-coding RNA generated by paired end RNA-seq technology. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of long-non coding RNA content within a cell. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biological functions. SOURCE: Leo Kurian (leo.kurian@uk-koeln.de) -  Center for Molecular Medicine Cologne

Pluto Bioinformatics

GSE115663: yylncT acts as a gatekeeper of the mesodermal transcriptional program by local modulation of DNMT3B [human_2]