We use Dimethyl Sulfate to probe the structure of rRNA and mRNA in yeast in vivo, in vitro, and at different temperatures in vitro. We obtain a great agreement between in vivo data and known mRNA structures as well as the ribosome crystal structure. We find that in contrast  to ribosomal rna, mRNAs are less structured in vivo than in vitro, and the structures present in vivo can only partially be explained by thermodynamic stability. In addition, we identify new regulatory structures present in vivo. SOURCE: Silvi Rouskin (silvirouskin@gmail.com) -  UCSF

Pluto Bioinformatics

GSE45803: Genome-wide RNA probing reveals mRNA structures are far less common in vivo than in vitro