Pluto Bioinformatics

GSE119487: m6A-seq profiling in WT, WtapKO and Mettl3KO mESCs

Bulk RNA sequencing

By m6A-seq, we examined the m6A pattern on Xist, which immediately after the A-repeat region. The cell line, integrating multiple copy of Xist on chr3, with m6A core machinery knockout (Wtap and Mettl3) were generated. We also profiled m6A-seq in transgenic Xist line, and observed a global reduction of m6A level, validated by m6A-LC-MS/MS. Unexpectedly, we havent observed reduced m6A level on Xist m6A region downstream of A-repeat in transgenic Xist line. SOURCE: Guifeng Wei (guifeng.wei@bioch.ox.ac.uk) - Brockdorff Lab University of Oxford