Pluto Bioinformatics

GSE135616: FBP1 loss disrupts liver metabolism and results in distinct transcriptome

Bulk RNA sequencing

This study set out to globally compare the liver transcriptomes from 24-week old wild type (WT) and FBP1-deficient mice. Total RNA was isolated from 10 snap frozen liver tissue samples using RNeasy Mini Kit (Qiagen). 250~300 bp insert eukaryotic mRNA derived cDNA non-directional libraryRNA libraries were prepared using standard Illumina protocols. The sequencing were performed on Illumina Platform PE150, with 20M raw reads/sample. SOURCE: Fuming Li (fumingli@pennmedicine.upenn.edu) - M. Celeste Simon University of Pennsylvania