Pluto Bioinformatics

GSE108699: On the design of CRISPR-based single cell molecular screens

Bulk RNA sequencing

We demonstrate that vector designs for such screens that rely on cis linkage of guides and distally located barcodes suffer from swapping of intended guide-barcode associations at rates approaching 50% due to template switching during lentivirus production, greatly reducing sensitivity. SOURCE: Jay Shendure (shendure@uw.edu) - Shendure Lab University of Washington