Pluto Bioinformatics

GSE100278: Next-generation sequencing measuring genome-wide RNA decay by s4U Seq in nave CD4+ T cells from Mettl3 WT and KO mice

Bulk RNA sequencing

Nave T cells of Mettl3 KO and WT were pulse labeled by s4U for 15 min before and after IL-7 stimulation for 15min/30min/45min/60min/75min/90min. The total input RNAs or s4U nascent RNAs were isolated and prepared for next-generation sequencing. The RNA response to IL-7 and RNA decay rates were then modeled and calculated, to infer the m6A/Mettl3 effects. SOURCE: Huabing Li (huabing.li@yale.edu) - Yale University School of Medicine