Pluto Bioinformatics

GSE127172: Gene expression profiles of mouse colonic epithelial monolayer cultured in air-liquid interface

Bulk RNA sequencing

The colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. Our understanding of how this process occurs is limited by a lack of in vitro models that recapitulate key aspects of in vivo responses. We established a long-term, self-organizing 2D epithelial monolayer system to model the cyclic nature of epithelial alterations during injury-repair. Through RNA-seq analysis, we seek to evaluate the transcriptomic profiles of the cultured epithelial cells under select phases of homeostasis-injury-repair in vitro. SOURCE: Thaddeus Stappnbeck (stappenb@wustl.edu) - Washington University School of Medicine