Purpose: Compare the RNA content of apoptotic human umbilical vein endothelial cells (HUVECs)-released vesicles: 1) Apoptotics bodies and 2) Apoptotic Exo-like vesicles, which are immunogenic and promote autoimmunity.;	Results: The transcriptome of ApoExos was unique with regards to its genomic origin, nucleotide composition and secondary structure. We show that ApoExos essentially contain non-ribosomal non-coding RNAs, among which 89% are derived from viral-like endogenous retroelements (EREs). ApoExos are also enriched in U-rich linear nucleotide sequences and Alus repeats.;	Conclusion: Our work demonstrates that apoptotic endothelial cells release EVs loaded with RNAs recognized by RLRs and endosomal TLRs (TLR3, TLR7 and TLR8), and are therefore tailor-made for initiation of innate immune responses and autoimmunity. SOURCE: Marie-Pierre Hardy (marie-pierre.hardy@umontreal.ca) - Claude Perreault University of Montreal

Pluto Bioinformatics

GSE119108: Apoptotic endothelial cells release nanovesicles loaded with immunostimulatory viral-like RNAs