Pluto Bioinformatics

GSE125509: Transcriptome profiling of H1-iCas9 cells grown on laminin and on MEFs

Bulk RNA sequencing

This experiment is part of a study of differentially essential genes in H1 hPSCs grown on different substrates ("Essential gene profiles for human pluripotent stem cells identify uncharacterized genes and substrate dependencies", Mair et al., Cell Reports 2018). The cells used stably express DOX-inducible Cas9 in the AAVS1 locus and were profiled together with unmodified H1 wt hPCS on both substrates to asses transcriptional changes. SOURCE: Jason Moffat (j.moffat@utoronto.ca) - University of Toronto